Composite

Part:BBa_M1031

Designed by: Neil Draper   Group: UtahState_BE5930_-_S11   (2011-04-27)


Total construct Pha, Phb, Phc plus zinc fingers and promoter

This is the entire construct of the DNA to be inserted into the plasmid and put into E. Coli. All Pha, Phb, and Phc enzymes are silver fusion compatible to their respective Zinc fingers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1257
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1903
    Illegal BglII site found at 3548
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2291
    Illegal NgoMIV site found at 4529
    Illegal AgeI site found at 138
    Illegal AgeI site found at 1663
    Illegal AgeI site found at 2760
    Illegal AgeI site found at 2847
    Illegal AgeI site found at 2991
    Illegal AgeI site found at 3264
    Illegal AgeI site found at 4047
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 496
    Illegal SapI site found at 625


[edit]
Categories
Parameters
None